Training Programme for Lab. Technician
*Total duration - 24 weeks
* There shall be three training sessions of 8 weeks each of which 6 weeks will be at the work place & 2 weeks hands-on training at the nominated Zonal Hospitals.
* Each training session to be followed by a suitable evaluation programme which will be conducted by the Pathologist Incharge/Zonal Hospital.
* Zonal Hospital of Northern & Southern Railway to be the nominated Hospitals for hands-on training & evaluation programme.
The course content and the mode of training for the three 6 weeks training session shall be as follows:
First 6 weeks training session
1. Anatomy And Physiology
a. Parts of human body-Anatomical terms and position, Different types of tissues and their identification.
b. Cardiovascularsystem- Circulation, Heart. Arteries, Veins, Bolld-composition, function, normal counts, coagulation and anticoagulants, CSF (composition, function), Grouping, identification of blood cells.
c. Urinary system-Kidney, Ureter, Bladder, Urethra, Prostate, Urine composition-normal and abnormal.
d. Nutrition- Carbohydrate, Protein, Fat, Vitamins and Minerals.
2. Haematology And Blood Banking
a. Introduction-Haematology, Collection of blood, Anticoagulants.
b. Haemoglobin estimation
c. RBC-Haemocytometer, calculation, abnormal forms, normal values, haematocrit, red. Cell indices, reticulocyte count, sickle cell preparation, anaemia.
d. WBC-Methods, calculation, immature forms, normal values, leukaemia.
e. Differential White cell count-Including staining procdure, eosinopphil count.
f. Erythorcyte Sedmantation Rate-Methods, values factors affecting ESR.
g. Coagulation tests-BT,CT,PT,APTT,Clot retraction test, Platelet count.
h. Preparation of bone marrow smears-All immature cells.
i. CSF examination
j. ABO blood group system
k. RH blood group system-Techniques of grouping.
l. Cross matching, combs test.
m. Blood transfusion-Procedure and complications.
n. Equipments-Principle & practice of equipments used in haematology & Bl.BK.
Two theory classes per week of 1 hour duration covering the above course shall be conducted during the entire 6 weeks session. Practical working covering the course content shall be supervised and monitored by the medical officer incharge during the routine laboratory working. The posting of the trainee during this session shall be in the haematology section of the laboratory.
Second 6 weeks training session
a. History-Micro organism in health and disease in man.
b. Serilization-Physical, Chemical and mechanical methods.
c. Bacteria-Identification, Different methods of staining, different types of cocci& bacilli.
d. Bacterial growth and cultivation of Micro-organism-Method, common culture and special media, antibiotic sensitivity tests.
e. Fungi- Morphology and cultivation.
f. Collection and processing-Sputum, urine, swabs, stool, blood, CSF and aspirations.
g. Semen analysis
h. Parasitology-Ova and cysts in stool.
i. Routine Examination of stool-Physical and microscopic examination.
j. Virology-General, HIV & Hepatitis.
k. Equipment-Theory and practical working of equipments used in microbiology.
a. Proteins- Theory and practical of total proteins, albumin, globulin & lipoprotein.
b. Carbohydrate- Theory and practical of glucose.
c. Lipids- Theory and practical of cholesterol, triglyceride, ketone bodies.
d. Liver function tests
e. Kidney function tests.
f. Collection and preservation-Blood, serum, plasma, CSF.
g. Electrolytes-Sodium, potassium and chlorides.
h. Equipments-Principle and practical working of equipments in biochemistry.
Two theory classes per week of 1 hour duration covering the above course shall be conducted during the entire 6 weeks session. Practical working covering the course content shall be supervised and monitored by the medical officer incharge during the routine laboratory working. The posting of the trainee during this session shall be in the microbiology and biochemistry section of the laboratory.
Third 6 weeks training session
1. Clinical Pathology, Serology & Immunology
a. Urine examination-Collection & preservation, Routine, microscopic, 24 hrs urine examination.
b. Stool examination
c. Sputum examination
d/ Semen analysis
e. Antogen and antibody reactions-Agglutination, widal, VDRL, RA test, CRP test.
f. Immunoassay-ELISA, Hormone &tumor marker study by different immunoassay methods.
g. Equipments-Principal & practice of equipments used in clinical pathology and immunology.
2. Histopathology and Cytology
a. Tissue Fixation-Different fixatives used in histopathology and cytopatholgy.
b. Tissue processing-Collection, labeling, fixation, dehydration, clearing, impregnation, embedding.
c. Section cutting-Microtome, technique of section cutting.
d. Staining-H&E, Giemsa, PAP.
e. Cytology-Types of specimens, preservation, Smear preparation and staining.
f. Equipments-Principle & practice of equipments used in histopathology & cytology.
3. Quality control in laboratory practice
4. Laboratory management and planning
Two theory classes per week of 1 hour duration covering the above course shall be conducted during the entire 6 weeks session. Practical working covering the course content shall be supervised and monitored by the medical officer incharge during the routine laboratory working. The posting of the trainee during this session shall be in the respective section of the laboratory.